李浩华; 陈玉婵; 陶美华; 李冬利; 潘清灵; 王磊; 章卫民
广东省微生物研究所广东省菌种保藏与应用重点实验室广东省微生物应用新技术公共实验室
【中文摘要】 以人肺癌细胞NCI-H460、中枢神经系统癌细胞SF-268及乳腺癌细胞MCF-7为供试细胞株,采用MTT(四甲基偶氮唑盐)法对毛蜂窝菌(Hexagona apiaria)野生子实体、栽培子实体、培养废料、发酵菌丝体的醇提物和发酵液乙酸乙酯萃取物、发酵液水相部分等各提取物进行了体外抗肿瘤活性研究。结果表明,毛蜂窝菌发酵液乙酸乙酯萃取物对三种肿瘤细胞株都有较明显的抑制作用,抑制率达80%以上;其它提取物也有一定的抑制作用,但抑制率在40%以下。
【英文摘要】 The anti-tumor activities of Hexagona apiaria extracts were evaluated with NCI-H460,SF-268 and MCF-7 cell lines using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium(MTT) method.Ethyl acetate extracts of the spent submerged culture medium significantly inhibited(>80%) all three tumor cell lines.Inhibitory effects were also observed with ethanolic extracts of both wild and artificially cultivated fruit bodies,spent compost after artificial cultivation,and fungal mycelium grown in submerged culture,as well as the aqueous phase remaining after ethyl acetate extraction of spent submerged culture medium,although the level of inhibition was <40% in all cases.
【中文关键词】 毛蜂窝菌; 提取物; 抗肿瘤活性; MTT法
【英文关键词】 Hexagona apiaria; extract; anti-tumor activity; MTT
【基金】广东省科技计划重点项目“食用菌产业推进关键技术研究与示范”(编号:2009B020201012)的部分研究内容
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2009年03期 【DOI】CNKI:SUN:SYJB.0.2009-03-014