雷萍; 黄志英; 林淼; 杨海华; 陈玉婷
上海市农业科学院农产品质量标准与检测技术研究所; 上海市饲料行业协会; 上海仪表电子工业环境监测站
【中文摘要】 本实验采用超高压高效液相检测法(UPLC)分离测定了多种虫草产品中腺苷和虫草素的含量。在最优化条件下,以ACQUITY UPLC C18为色谱柱,流动相为乙腈和水,等度洗脱,上述各组分在4 min内可基本实现基线分离。各组分浓度与峰面积在117.5μg/mL范围内呈良好线性,检测下限(S/N=3)分别为0.10μg/mL和0.12μg/mL。采用该方法成功测定了多种虫草产品中腺苷和虫草素的含量。
【英文摘要】 Adenosine and cordycepin levels in twelve Cordyceps products were successfully determined by Ultrahigh-Performance Liquid Chromatography(UPLC) using an ACQUITY C18 column,with water and acetonitrile as the mobile phase.Separation of the two components was achieved within four minutes,and the concentrations of each component showed a linear relationship with the peak area in the range 2～20 μg/mL.Detection limits(S/N=3) were 0.10 μg/mL and 0.12 μg/mL for adenosine and cordycepin,respectively.
【中文关键词】 UPLC; 腺苷; 虫草素
【英文关键词】 Ultrahigh-Performance Liquid Chromatography; adenosine; cordycepin
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2009年03期 【DOI】CNKI:SUN:SYJB.0.2009-03-016