YING Zheng-he 1; 2; WU Xiao-ping 1; XIE Bao-gui 1*; CHEN Li-na 1; LU Qi-quan 1; GAO Wei 1; SUN Shu-jing 1 1Mycological Research Center; Fujian Agriculture and Forestry University; Fuzhou; Fujian 350002; China; 2 Institute of Plant Protection; Fujian Academy of Agriculture Sciences; Fuzhou; Fujian 350013; China
【英文摘要】 A stable Sequence-Related Amplified Polymorphism (SRAP) molecular marker system for Lentinula edodes has been developed. A high number of amplification products and good reproducibility was achieved using a 25 μL reaction system consisting of: 2.5 mM Mg 2+, 250 μM dNTP mix,1.5 U Taq DNA polymerase,0.5 μM primers,20 ng template DNA and 2.5 μL 10×PCR buffer.
【英文关键词】 Lentinula edodes; SRAP; System optimization
【基金】Sponsored by the Foundation of Fujian Province(2006S1001)
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2006年04期 【DOI】CNKI:SUN:SYJB.0.2006-04-001