王晓洁; 蔡德华; 杨立红; 卜庆梅
烟台师范学院食用真菌研究所; 烟台师范学院食用真菌研究所; 烟台师范学院食用真菌研究所; 烟台师范学院食用真菌研究所 山东烟台 264025 ; 山东烟台 264025 ; 山东烟台 264025 ; 山东烟台 264025
【中文摘要】 通过深层发酵获得金顶侧耳菌丝体。用水提法分别提取金顶侧耳菌丝体多糖、胞外(过滤液)多糖和全液(菌丝体+发酵液)多糖。用MTT比色法测定金顶侧耳多糖体外对小鼠S-180癌细胞及人结肠低分化腺癌细胞的抑制率。结果表明,金顶侧耳胞外多糖对体外培养的S-180癌细胞有抑制作用,全液多糖和菌丝体多糖无抑制作用。这3种多糖体外对人结肠低分化腺癌细胞有抑制作用,其中金顶侧耳胞外多糖抑制率最高,全液多糖次之,菌丝体多糖最低。
【英文摘要】 The mycelia of Plearotus citrmopileatus were cultured through submerged fermentation, and po-lysaccharides were extracted from the mycelia, the filtrate and the fermented broth resp. . While the inhibition rate of P. citrinopileatus polysaccharide for mouse S-180 tumour cells and low-differentiated adenocarcinoma cells of human colon were determined by MTT colorimetry. The experiment results showed that the excellular polysaccharide extracted from the filtrate was proved to have inhibition effect to cultured S-180 cells in vitro, the polysaccharide extracted from the mycelia and the fermented broth have no inhibition effect to cultured S-180 cells in vitro. And it was also proved in our experiment that three kinds of P. citrinopileatus polysaccharide all had antitumour activity to cultured low-differentiated adenocarcinoma cells of human colon in vitro , and that the inhibition rate of the polysaccharide extracted from the filtrate was the highest, followed by the polysaccharide extrac...更多ted from the fermented broth and the mycelia of P. crtrinopileatus. 还原
【中文关键词】 深层发酵法; MTT比色法; 水提法; 金顶侧耳; 多糖; 抑肿瘤作用
【英文关键词】 Submerged fermentation; MTT colorimetry; Water extraction; Pleurotus citrinopileatus; Po-lysaccharide; Antitumor activity
【基金】山东省科技厅攻关项目"食用菌液体深层培养技术应用研究"(011100108)的部分内容
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2005年01期 【DOI】CNKI:SUN:SYJB.0.2005-01-003