刘传富; 皇传华; 黄春燕; 单洪涛; 赵传麟
济南市农业科学研究所; 济南市农业科学研究所; 山东省农科院植保所; 山东省农科院植保所; 济南市农业科学研究所 济南250023 ; 济南250023 ; 济南250100 ; 济南250100 ; 济南250023
【中文摘要】 研究结果表明,培养鸡腿蘑液体菌种的适宜培养基配方为蔗糖2%,玉米粉3%,麦麸4%,酵母粉0.5%,KH2PO40.25%,MgSO4·7H2O0.1%,pH6.2;最适培养条件是250mL三角瓶装液量60mL,接种量5%,摇床转速120r min,培养6d。利用液体菌种扩繁固体生产种,可以明显缩短菌种生产周期。
【英文摘要】 The experimental results showed that the optimum medium of Coprinus comatus liquid spawn was sucrose 2%,corn powder 3%,wheat bran 4%,yeast powder 0.5%,KH_2PO_4 0.25%,MgSO_4·7H_2O 0.1%,and that the optimum culture conditions were as follows: initial pH6.2,medium capacity 60mL/250mL flask, rocking bed rotation rate 120r/min, inoculation rate 5% and the culture period of about 6 days. And the culture period will be shortened significantly if liquid spawn was used to breed the solid culture spawn.
【中文关键词】 鸡腿蘑; 液体菌种; 培养条件; 正交试验
【英文关键词】 Coprinus comatus; Liquid spawn; Culture condition; Orthogonal test
【基金】山东省济南市科技局"食用菌菌种引进选育及研究"[济科合字(2001)第70-1号]的部分内容
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2004年02期 【DOI】CNKI:SUN:SYJB.0.2004-02-005