张丽霞; 郭成金
天津师范大学化学与生命科学学院
【中文摘要】 采用L16(45)正交设计,得出猪苓原生质体制备最佳条件是取菌龄7d的菌丝体,以2%溶壁酶,0.6mol·L-1甘露醇作为渗透压稳定剂,在33℃下酶解3h,原生质体数达到2.62×107个·mL-1。原生质体再生的最佳条件是取菌龄5d的菌丝体,以2%溶壁酶,0.6mo·lL-1蔗糖作为渗透压稳定剂,在27℃的条件下,酶解3h。猪苓原生质体再生的最佳培养基为葡萄糖10g、麦芽糖5g、酵母粉4g、0.6mo·lL-1甘露醇、琼脂5.5g,定容到1000mL。再生率可达到0.65%。
【英文摘要】 Author adopted orthogonal experiment and obtained the results.The optimal preparing condition of Grifola umbellata protoplast is that 7-day-old mycelium,applying 0.6mo·lL-1mannitol for osmotic pressure stabilizer,using 2%lywallzyme,digesting at 33℃for 3 hours.The number of protoplast is 2.62×10·7mL-1.Protoplasts produced in the condition that 5-day-old mycelium,at 27℃ by 0.6mol·L-1 cane sugar,2%lywallzyme shacking 3 hours were optimal to regeneration.The medium with the following composition:glucose 10g,yeast extracts 4g,maltose 5g,agar 5.5g,0.6mol·L-1 mannitol,added distilled water to 1000mL was suitable for regeneration.The regeneration rate was 0.65%.
【中文关键词】 猪苓; 原生质体; 正交设计; 制备; 再生
【英文关键词】 Grifola umbellata; Protoplast; Orthogonal design; Preparation; Regeneration
【基金】天津市科学技术委员会项目(编号:06ZHCXNC05900)
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,2008年05期 【DOI】CNKI:SUN:ZSYJ.0.2008-05-018