胡文彬; 马海乐; 周存山
江苏大学生物与环境工程学院; 江苏大学生物与环境工程学院; 江苏大学生物与环境工程学院 镇江212013; 镇江212013; 江苏省农产品生物加工与分离工程技术研究中心; 镇江212013; 镇江212013
【中文摘要】 研究了桑黄菌在发酵过程中菌丝体及胞内外多糖产量变化趋势,碳源、氮源以及接种量、摇床转速、温度和pH值等因素对桑黄菌液体深层发酵菌丝产量的影响。结果表明:玉米粉为最佳碳源,豆饼粉为最佳氮源,优化培养基配方为:玉米粉4.0%、豆饼0.5%、KH2PO40.1%、MgSO4.7H2O0.05%;最适菌丝体生长的液体发酵条件:培养温度26℃,摇瓶转速130rpm,pH值6.5,接种量15%￣20%,培养时间7d。
【英文摘要】 The change tendency of output from intracellular and extracellular polysaccharide in the fermentative period for the mycelium of Phellinus igniarius was studied.Carbon source,nitrogen source,inoculating amount,rotational speed,temperature and pH etc.affected the output of mycelium in liquid submerged fermentation for Phellinus igniarius.The results showed that maize meal was the optimum carbon source,and bean cake was the best nitrogen source.The fine formula of medium was: maize meal 4.0%,bean cake 0.5%,KH2PO40.1%,MgSO4·7H2O0.05%.The most suitable fermentative condition was: cultivated temperature 26℃,rotational speed130rpm,pH6.5,inoculating amount 15%20%,growth time 7d.
【中文关键词】 桑黄; 深层发酵; 菌丝产量; 多糖
【英文关键词】 Phellinus igniarius; Submerged fermentation; Mycelium yield; Polysaccharide
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,2006年03期 【DOI】CNKI:SUN:ZSYJ.0.2006-03-017