程东升; 潘学仁
东北林业大学
【中文摘要】 过氧化物酶同工酶（ＰＯＤ）是真菌遗传分析中的一个常用生化指标，在其电泳分析中染色操作是一个重要技术环节。本研究以香菇、美味侧耳为材料，比较了不同ＰＯＤ染色方法的染色效果。ＮＨ＿３Ｃｌ－联苯胺法效果最佳，醋酸一联苯胺法次之，ＡＣ－联苯胺及愈创木酚－联苯胺法效果较差。对影响染色效果的原因进行了分析。
【英文摘要】 Peroxidase isozymes (POD) are one of the frequently-used biochemical markers in genetical analysis of fungi,The staining is an important procedure in studying POD.Theefficiency was compared;among different stain recipes for POD of two edible fungi Lentinus edodes and Pleurctus sapidus. The recipe NH_3Cl-Benzidine showed the best effect,and followed the HAc-Benzidine,but the Vc-Benzidine and Guaiacol-Benzidine did not gave satisfactory results,The possible reasons for differences of stain effect were discussed.
【中文关键词】 食用菌; 过氧化物酶同工酶; 染色方法
【英文关键词】 Edible fungi; Peroxidase isozyme; staining methods.
【文献出处】 中国食用菌,EDIBLE FUNGI OF CHINA,编辑部邮箱,1994年03期 【DOI】CNKI:SUN:ZSYJ.0.1994-03-006