【作者】 田雪莲;陆伟东;丁伟;卯霞;田雪珊;张军
【Author】 TIAN Xue-lian1,LU Wei-dong1,DING Wei1,MAO Xia1,TIAN Xue-shan2,ZHANG Jun1(1.Yun-gui Plateau Institute of Biodiversity,Qujing Normal University,Qujing Yunnan 655011;2.Qujing Disease Control Center,Qujing Yunnan 655000)
【机构】 曲靖师范学院云贵高原生物多样性研究所;曲靖市疾控中心
【摘要】 通过对Pm0217F菌株进行不同时间梯度的紫外诱变,得到5株不同的诱变菌株。通过对5个诱变株的菌落特征、生长速率、大孢子千分率、分生孢子不同聚集方式及虫草菌素含量等生物学特性比较,结果显示,诱变最适时间为60s,且大孢子比例高、拟青霉型产孢结构比例大的菌株生物学特性较优。
【Abstract】 Ultraviolet radiation to mutate Pm0217F were adopted,and five different strains were obtained at different times of UV mutagenesis.The colony morphology,growth rate,megaspore's permillage,conidiophore of different aggregation methods and content of cordycepin were compared.The results showed that the best time was 60 s,and the strain that had high proportion of megaspore and high proportion of Paecilomyces-type showed the best biological characters.
【关键词】 蛹虫草;分生孢子;虫草菌素;拟青霉型
【Key words】 Cordyceps militaris;Conidiospore;Cordycepin;Paecilomyces-type
【基金】 曲靖师范学院科技创新团队基金资助项目
【文献出处】 中国食用菌, Edible Fungi of China, 编辑部邮箱 , 2010年05期
【DOI】CNKI:SUN:ZSYJ.0.2010-05-006