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Isolation, Purification and Immunological Activities of a Polysaccharide Fraction from Agrocybe aege

2010-05-17 09:301630

FANG Yi-hong; YU Ping*; LI Wei; LU XunCollege of Chemistry and Material Science; Fujian Teachers University; Fuzhou 350007; China

【英文摘要】 A plysaccharide fraction, ACPS, was obtained from fruit bodies of Agrocybe aegerita following extraction with hot water, protein removal by the Sevage method, ethanol precipitation, and Sephadex G-100 and DEAE-Sephadex chromatography. Analysis of the chemical structure of ACPS by thin-layer chromatography, HPLC and IR spectrometry revealed an acidic polysaccharide with a molecular mass of 11863. The polysaccharide, reducing sugar and uronic acid contents were 91.09%, 10.05% and 4.73%, respectively. Intraperitoneal administration of ACPS to mice significantly increased the spleen weight, the phagocytic rate and phagocytic index, thereby indicating a promoting effect on the immune function.

【英文关键词】 Agrocybe aegerita; Fruit body polysaccharide; Immunological activitiy; Isolation and purification
【基金】Sponsored by the Foundation of Fujian Education Office(No:JA02185)

【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2006年04期 【DOI】CNKI:SUN:SYJB.0.2006-04-015

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