徐朝晖; 姜世明; 付培武
山东师范大学和物系; 山东省动物抗性重点实验室
【中文摘要】 双孢蘑菇子实体（Ａｇａｒｉｃｕｓｂｉｓｐｏｒｕｓ）经热水提取，乙醇沉淀，胰蛋白酶消化，Ｓｅｖａｇ法去除蛋白质等处理，得多糖。经紫外可见光分光光度计测定无紫外吸收峰，为较纯多糖。并研究了其对人肝癌ＳＭＭＣ－７７２１细胞生长曲线、有丝分裂指数及线粒体活性的影响，结果表明该多糖对体外培养的人肝癌ＳＭＭＣ－７７２１细胞增殖有一定抑制作用，抑癌实验证明该多糖对小鼠Ｓ－１８０实体瘤亦有一定抑制作用。
【英文摘要】 The polysacchride was isolated from the fruit bodies of Agaricus bisporus Sing. and verified by ultraviolet spectrum. The proliferation of human hepatoma SMMC-7721 cell line was significantly inhibited by the treatment of 50ug/mL the polysacchride. The conclusion was proved by determining the variation of the growth curve, mitosis index and mitochodria activity. The inhibitory activity of the polysacchride against implanted S-180 tumor in mice was also perfromed with the 51.9% inhibition.
【中文关键词】 双孢蘑菇子实体; 多糖; 抑瘤
【英文关键词】 Agaricus bisporus Sing; polysacchride
【基金】山东省科委资助
【文献出处】 中国食用菌,EDIBLE FUNGI OF CHINA,编辑部邮箱,1997年04期 【DOI】CNKI:SUN:ZSYJ.0.1997-04-001