【作者】 孙晓红； 陈明杰； 汪虹； 冯爱萍； 潘迎捷；
【Author】 SUN Xiaohong1,2,3,CHEN Mingjie1,WANG Hong1,FENG Aiping1,PAN Yingjie3 1National Engineering Research Center of Edible Fungi;Key Laboratory of Applied Mycological Resources and Utilization,Ministry of Agriculture;Shanghai Key Laboratory of Agricultural Genetics and Breeding;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China;2College of Life Sciences,Nanjing AgriculturalUniversity,Nanjing,Jiangsu 210095,China;3Shanghai Ocean University,Shanghai 200090,China
【机构】 国家食用菌工程技术研究中心农业部应用真菌资源与利用重点开放实验室上海市农业遗传育种重点开放实验室上海市农业科学院食用菌研究所； 南京农业大学生命技术学院； 上海海洋大学；
【摘要】 根据草菇(Volvariella volvacea)S-腺苷-L-高半胱氨酸水解酶基因(sahh)全长序列设计两个嵌套引物,与Takara LA PCR in vitro cloning试剂盒的接头引物结合进行巢式PCR扩增,得到sahh上游调控序列,通过序列分析发现,该序列含有1个TATA盒和4个CAAT盒。应用MatInspector程序分析sahh上游调控序列,发现该区域存在1个耐盐因子,1个热激因子,2个DRE元件和2个ABA应答元件。
【Abstract】 An upstream regulatory sequence of a S-adenosyl-L-homocysteine hydrolase gene(sahh) from Volvariella volvacea was amplified by nested PCR using two nested primers designed according to the full-length sequence of sahh and which were connected to the primer linker of the Takara LA PCR in vitro cloning box.The upstream sequence contained one TATA box and four CAAT boxes.MatInspector program analysis revealed one salt resistance factor,one heat shock factor,two drought-response binding factors(DRE)...
【关键词】 S-腺苷-L-高半胱氨酸水解酶基因； 调控序列； 草菇；
【Key words】 Volvariella volvacea； S-adenosyl-L-homocysteine hydrolase gene； regulatory sequence；
【基金】 农业部公益行业科研专项(编号:nyhyzx07-008)的部分研究内容
【分类号】S646.13