林汝楷; 傅俊生; 上官舟建
福建省三明真菌研究所; 福建农林大学菌物研究中心
【中文摘要】 采用随机扩增多态性DNA(Randomly Amplified Polymorphic DNA,RAPD)技术分析玉木耳(whitemutant strain ofAuricularia fuscosuccinea)、九里湖木耳(A.fuscosuccinea)、黑木耳(A.auricula)8129和毛木耳(A.polytricha)781四个菌株之间的遗传差异性。结果表明,玉木耳与九里湖木耳之间的遗传相似系数大于0.99;4个菌株在遗传距离0.53的位置上可以分为三类:九里湖木耳和玉木耳为一类;黑木耳8129,毛木耳781各为一类。
【英文摘要】 Genetic variation among a white mutant strain of Auricularia fuscosuccinea,its parent A.fuscosuccinea strain,A.auricula(strain 8129) and A.polytricha(strain 781) were analyzed by RAPD(Randomly Amplified Polymorphic DNA).No genetic differences were detected between the white mutant strain and the A.fuscosuccinea parent strain(genetic similarity coefficient < 0.99),and the four strains could be separated into three sub-groups at a genetic distance of 0.53.The white mutant strain together with its parent strain formed one sub-group,and the other two sub-groups were made up of A.auricula strain 8129 and A.polytricha strain 781,respectively.
【中文关键词】 玉木耳; 白色变异菌株; 随机扩增多态性DNA(RAPD)
【英文关键词】 Auricularia fuscosuccinea; white mutant strain; Randomly Amplified Polymorphic DNA(RAPD)
【基金】福建省重点科技计划项目“玉木耳选育与工厂化栽培技术研究”(编号:2004N058);; 三明市科技计划项目“玉木耳人工栽培研究”(编号:2001-农-7);; 福建省科技创新平台建设项目“福建省食(药)用菌种质资源科技公共服务平台建设及相关技术研究”(编号:2006S1001)的部分研究内容
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2008年02期 【DOI】CNKI:SUN:SYJB.0.2008-02-004