TAN Qi 1 ; ROMAINE CP 2 ; SCHLAGNHAUFER C 2 ; CHEN Ming-jie 1 ; GUO Qian 1 ; GEML J 2 and GARZON C 2 1 Institute of Edible Fungi; Shanghai Academy of Agricultural Sciences; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Shanghai 201106; P.R. China; 2 Department of Plant Pathology; The Pennsylvania StateUniversity; University Park; Pennsylvania 16802; USA
【英文摘要】 The distribution of a 621 bp sequence of the ITS region of nuclear rDNA, and a 519 bp fragment of the protein-coding translation elongation factor gene (EF-1α), among six commercial strains of Pleurotus nebrodensis collected from the main cultivation area of China confirmed that the six strains belonged to the same species. RAPD data indicated that five strains were very closely related, with character differences rated at or below 3.4% using the UPGMA method of PAUP 4.0. Character differences between P. nebrodensis strain No.6 and the five other strains were rated at 16.9%, and a specific primer for this strain has been identified.
【英文关键词】 Pleurotus nebrodensis; ITS; Protein-coding translation elongation factor gene (EF-1α); RAPD; Specific primer
【基金】theFoundationofShanghaiAgricultureCommittee(No.2000-1385)
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2006年01期 【DOI】CNKI:SUN:SYJB.0.2006-01-003