袁丽铭; 忻茜; 李兴玉
上海师范大学生命与环境科学学院; 上海师范大学生命与环境科学学院; 上海师范大学生命与环境科学学院 上海200234 ; 上海200234 ; 上海200234
【中文摘要】 应用流式细胞分选仪(Flow cell sorter,FCS)和透射电子显微镜(Transmissional elec-tron microscope,TEM)等技术观察了巴西蘑菇(Agaricus blazei)多糖诱导K562肿瘤细胞凋亡的试验研究。试验发现,流式细胞仪可检测到经巴西蘑菇多糖诱导K562肿瘤96h后产生明显的亚二倍体峰;电镜下观察到K562肿瘤细胞核固缩,核内染色质浓缩、凝聚,紧靠在细胞核膜边沿,形成新月状或环状结构等典型的凋亡细胞的形态变化及凋亡小体。结果表明,巴西蘑菇多糖具有诱导K562肿瘤细胞凋亡的作用。
【英文摘要】 The research on the apoptosis of K562 tumor cells induced by polysaccharide extracted from Agaricus blazei was carried out using advanced technology of flow cell sorter and transmissional electron microscope.The result showed that,visible apoptotic peak could be detected by flow cell sorter after being induced for 96h by polysaccharide extracted from Agaricus blazei.Furthermore,karyopyknosis,chromatin condensed and aggregated,hugging the edge of cell nucleus,showing crico-structure,crescent-structure and other typical morphologic changes of apoptotic cell as well as apoptotic body could be seen in K562 cells through electro-microscope.It was concluded that the apoptosis of K562 tumor cells could be induced by polysaccharide extracted from Agaricus blazei.
【中文关键词】 巴西蘑菇多糖; 诱导; K562肿瘤细胞; 细胞凋亡
【英文关键词】 Polysaccharide extracted from Agaricus blazei; Induce; K562 tumor cell; Apoptosis
【基金】上海市高等院校科学技术发展基金项目“巴西蘑菇DNA反义核酸抗肿瘤的实验研究”(编号:02DK09)的部分研究内容
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2005年03期 【DOI】CNKI:SUN:SYJB.0.2005-03-007