汪虹; 瞿伟菁; 曹群华
上海市农业遗传育种重点实验室上海市农业科学院食用菌研究所; 华东师范大学生命科学院; 华东师范大学生命科学院 上海201106 ; 上海200062 ; 上海200062
【中文摘要】 研究表明,金耳(Tremellaaurantialba)液体发酵最适培养基组成为玉米粉2%,蔗糖3%,酵母膏0.1%,KH2PO40.1%,MgSO40.05%;培养基最佳初始pH、250mL三角瓶装量、接种量、种子菌龄分别是3.5、80mL、10%和120h;用10L发酵罐发酵时,最佳培养条件是种子种龄144h、28℃、接种量10%、通风量2.5L min。
【英文摘要】 The results of orthogonal test showed that the optimum medium for the mycelial growth of Tremella aurantiabla was composed with 2% corn powder, 3% sucrose, 0.1% yeast extract, 0.1% KH_2PO_4 and 0.05% MgSO_4,and that the optimum initial pH, medium capacity of 250mL Erlenmeyer flask, inoculation rate and spawn age were 3.5, 80mL, 10% and 120h resp.. When the mycelia of Tremella aurantiabla were cultured in 10L fermentor, the optimum spawn age, culture temperature, inoculation rate and ventilation rate for the mycelial growth of Tremella aurantiabla were 144h, 28℃, 10% and 2.5L/min resp..
【中文关键词】 金耳; 液体发酵; 正交试验; 菌丝产量
【英文关键词】 Trmella aurantialba; Submerged culture; Orthogonal test; Mycelial yield
【基金】上海市高等学校科研基金资助项目(JG9507)
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2003年04期 【DOI】CNKI:SUN:SYJB.0.2003-04-007