王淑珍; 白晨; 范俊; 高雁; 扬家峰; 扬英杰
上海师范大学生命与环境科学学院; 复旦大学生命科学学院; 上海师范大学生命与环境科学学院; 上海师范大学生命与环境科学学院; 上海师范大学生命与环境科学学院; 上海师范大学生命与环境科学学院 上海200234 ; 上海200433 ; 上海200234 ; 上海200234 ; 上海200234 ; 上海200234
【中文摘要】 以获得弱木栓质化灵芝菌株为目的 ,利用液体培养 4d的灵芝菌丝体和培养 3d的糙皮侧耳菌丝体为材料 ,在PEG Ca2 +系统中诱导原生质体融合 ,获融合子 19株 ,融合率为 1.9×10 -5。根据其生物学特性 ,选取F3、F8两融合子及两亲本菌株的基因进行RAPD分析。结果表明 ,两亲本的核物质在融合子中发生了重组 ,并影响了酶蛋白的表达 ,而融合子在DNA水平上更接近灵芝
【英文摘要】 In order to obtain less corkification strain of G.lucidum, the mycelia of G.lucidum and P.ostreatus were put under submerged culture for 4d and 3d respectively to prepare the protoplast. The fusion experiment between G. lucidum and P. ostreatus protoplasts was carried out under PEG Ca 2+ system, and nineteen fusants were selected.The fusion frequency was as high as 1.9×10 -5 . According to their biological characters, RAPD analysis for genomes of fusants F3?F8 selected randomly and two parents was conducted. The results showed that the nuclear substance of two parents was recombined in the fusant and the expression of the enzyme protein was affected. The fusants were proved to be more similar to G.lucidum at DNA level.
【中文关键词】 灵芝; 糙皮侧耳; 原生质体; 融合; 融合子; RAPD分析; DNA谱带
【英文关键词】 Ganoderma lucidum; Pleurotus ostreatus; Protolast; Fusion; Fusant; RAPD analysis; DNA band.
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2003年01期 【DOI】CNKI:SUN:SYJB.0.2003-01-000