彭卫红; 肖在勤; 郑林用; 甘炳成
四川省农业科学院土壤肥料研究所; 四川省农业科学院土壤肥料研究所; 四川省农业科学院土壤肥料研究所; 四川省农业科学院土壤肥料研究所 成都610066 ; 成都610066 ; 成都610066 ; 成都610066
【中文摘要】 以长根菇和鸡【为亲本进行属间不对称融合研究 ,从原生质体再生菌株中得到能够双核化的单核无锁状联合的菌株 2 0株。栽培结果显示 ,这类菌株不能形成子实体 ,且菌株间菌丝的生长差异显著。但在特定的培养条件下 ,单核菌株均可双核化形成双核菌株 ,双核菌株具有出菇能力 ,菌株间子实体颜色、菌柄着生方式及菌丝生长速度等与亲本有明显的差异
【英文摘要】 Through asymmetric fusion between O.radicata and T.albuminosus under high pH and high Ca 2+ conditions, 20 monokaryotic strains without clamp connection which could be dicaryotized were obtained from regenerative protoplast strains. The cultural test indicated that no fruitbody was developed from monokaryotic strains and the mycelium growth characteristics between monokaryotic strains were significantly different. Under given conditions, the monokaryotic strains were dicaryotized into dicaryotic strains which could form fruitbody. But the fruitbody colour, the stipe insertion mode and the mycelium growth rate of dicaryotized strains were significantly different from their parent strains and also significantly different between the dicaryotic strains tested.
【中文关键词】 长根菇; 鸡【; 不对称融合; 生长特性; 双核化
【英文关键词】 Oudemansiella radicata; Termitomyces albuminosus; Asymmetric fusion; Growth characteristic; Dicaryotization
【基金】农业部重点资助项目 (99-农 - 0 6 )
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2002年01期 【DOI】CNKI:SUN:SYJB.0.2002-01-000