陈明杰; 余智晟; 凌霞芬; 王南; 谭琦; 吕作舟; 潘迎捷
农业部食用菌遗传育种重点开放实验室!上海市农业遗传育种重点开放实验室; 上海市农业科学院食用菌研究所; 上海201106; 华中农业大学微生物系应用真菌室!武汉430070; 农业部食用菌遗传育种重点开放实验室!上海市农业遗传育种重点开放实验室; 上海市农业科学院食用菌研究所; 上海2011
【中文摘要】 利用现代分子生物学技术扩增获得草菇组织分离物与草菇出发菌株的RAPD和ITS的DNA指纹图谱 ,以此估算两者的DNA相似系数 ,并构建了遗传相关聚类图。结果表明 ,两者存在着一定的遗传差异。
【英文摘要】 The amplified DNA fingerprints from RAPD and ITS of Volvariella volvacea tissue isolates and parental strains were obtained by modern molecular biological techniques. The DNA similarity coefficients of V. volvacea strain and tissue isolate were estimated and cluster dendrogram was constructed based on the DNA fingerprints. The results showed that genetic variation existed between these two strains.
【中文关键词】 草菇; 组织分离; 遗传变异
【英文关键词】 Volvariella volvacea; Tissue isolate; Genetic variatio(
【基金】上海市农委科技兴农重点攻关项目
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2000年02期 【DOI】CNKI:SUN:SYJB.0.2000-02-000