何志勇; 王镨; 徐晋麟; 张惟杰
上海交通大学生命科学与技术学院!上海200240; 浙江工业大学轻工系!杭州310014; 上海交通大学生命科学与技术学院!上海200240; 上海交通大学生命科学与技术学院!上海200240
【中文摘要】 对融合子F2的培养条件进行了正交试验 ,着重对温度、培养基 pH、碳源种类和氮源浓度进行了优化。结果表明 ,35℃、pH6.0、可溶性淀粉、2 %(NH4 ) 2 SO4 为其最适培养条件。其最适 pH与两个亲本的基本相近 ,其最适生长温度接近虎皮 - 0 1亲本的生长温度 ,这为融合子能在较高温度下生长和出菇提供了条件
【英文摘要】 The culture conditions such as temperature, pH, carbon source and nitrogen source for fusant F2 were optimized. It was proved in our test that 35℃,pH6.0,soluable starch (as the carbon source) and 2% (NH 4) 2SO 4 (as the nitrogen source) were the optimal culture conditions for F2, that the optimal pH for the medium was almost the same as that of two parent strains, while the optimal temperature was the same as that of the parent Lentinus tigrinus strain and much higher than that of the parent L.edodes strain (25℃), and that fusant F2 could form fruitbody at high temperature.
【中文关键词】 原生质体融合菌株; 正交试验; 优化
【英文关键词】 Protoplast fusant; Orthogonal experiment; Optimization
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2000年01期 【DOI】CNKI:SUN:SYJB.0.2000-01-002