陈再鸣; 江家余; 王涛
浙江农业大学食用菌研究所; 浙江农业大学食用菌研究所; 浙江农业大学食用菌研究所 杭州 310029 ; 杭州 310029 ; 杭州 310029
【中文摘要】 研究了51株侧耳属(Pleurotus)菌株的菌丝体形态学、细胞学和生理生化特征,比较了它们与菌株的产量性状、质量性状和抗性水平之间的关系。结果发现,在平皿中菌丝生长速度与子实体品质之间呈较密切的负相关。菌丝体胞外CMC酶、TTC-脱氢酶活性与菌株的产量性状呈正相关。POD同工酶谱间的异同可反映菌株间抗性水平的差异。根据菌落形态和菌丝体细胞结构的变化,色素分泌及无性孢子产生,结合EST、POD同工酶谱带变化,可初步进行种性变异的检测。
【英文摘要】 A study on the relationship of mycelial morphological, cytological and physiological-biochemical characteristics of 51 Pleurotus strains with the characteristics of the yield, quality and resistibility of these strains was conducted. The results showed that there was a quite close negative correlativity between the mycelial growth rate and the fruitbody quality of Pleurotus strains cultured on plate; that there was a positive correlativity between the activity of mycelial extracellular CMCase, TTC-dehydrogenase and the yield performance of Pleurotus strains, and that the difference of POD isozyme zymogram could reflect the resistibility difference among strains. And the species variance could primarily be investigated according to the change of colony morphology and mycelium cell structure, the pigment secretion and the conidium formation accompanied with the zymogram band change of EST and POD isozyme.
【中文关键词】 侧耳属; 菌株; 生长特征; 种性; 相关性
【英文关键词】 Pleurotus: Strain; Growth characteristic; Species property; Correlativity
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,1998年01期 【DOI】CNKI:SUN:SYJB.0.1998-01-003