冯伟林; 蔡为明; 金群力; 范丽军
浙江省农业科学院园艺所; 浙江大学农业与生物技术学院
【中文摘要】 试验应用ISSR技术分析了杏鲍菇生产性菌株的DNA序列多态性,从20个引物中筛选出11个ISSR引物对12个杏鲍菇生产性菌株基因组DNA进行PCR扩增。结果表明,这些杏鲍菇菌株遗传相似系数为0.68～1.00。采用平均分类法UPGMA分析表明,在遗传相似系数为0.83处可将这12个杏鲍菇生产性菌株分成3类。
【英文摘要】 Inter-simple sequence reqeats (ISSR) methods were used to analyze the DNA sequence polymorphism of cultivated Pleurotus erngii strains. 11 primers were chosed for ISSR among 20 primers. Genomic DNA of Pleurotus erngii were amplified by 11 primers using PCR. The results showed that the genetic similar coefficients (GSC) were 0.681.00. Cultivated strains were divided into 3 groups at GSC 0.83 based on the cluster analysis of UPGMA method.
【中文关键词】 杏鲍菇; 遗传差异; ISSR
【英文关键词】 Pleurotus erngii; Genetic variation; ISSR
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,2009年01期 【DOI】CNKI:SUN:ZSYJ.0.2009-01-022