杨梅; 王丽雅; 庄跃飞; 陈凌华
福建师范大学生物工程学院; 福建师范大学生物工程学院; 福建师范大学生物工程学院; 福建师范大学生物工程学院 福州350007 ; 福州350007 ; 福州350007 ; 福州350007
【中文摘要】 杏鲍菇是一种珍贵食用菌,杏鲍菇多糖具有多种生物活性,本文对杏鲍菇菌丝球样品进行热水提取,去蛋白,乙醇沉淀,无水乙醇离心,真空干燥,得到多糖粗品。再用DEAE—纤维素层析法进行多糖的分离,梯度洗脱收集,采用硫酸—苯酚法测定多糖,结果得到三个主要糖峰。
【英文摘要】 Pleurotus eryngii is a kind of rare and edible fungus.Its polysaccharides have various biological activities.In the paper,some results of the extraction and isolation of polysaccharides from the mycelia of Plcurotus eryngii were reported.Firstly after being dried and smashed,the mycelia was extracted with hot water.Then the raw polysaccharides were obtained by means of ethanol precipitation,protein removal by sevage method.The raw polysaccharide were purified with column chromatography on DEAE-cellulose and collected by cleansed.The existence of three peaks of polysaccharides was determined by phenol-sulphuric acid.
【中文关键词】 多糖; 提取; 分离; 分析
【英文关键词】 Polysaccharides; extraction; isolation; analysis
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,2005年04期 【DOI】CNKI:SUN:ZSYJ.0.2005-04-015