张平; 陈松; 陈作红
湖南师范大学生命科学学院; 湖南师范大学生命科学学院; 湖南师范大学生命科学学院 长沙410081 ; 长沙410081 ; 长沙410081
【中文摘要】 致命鹅膏 (Amanitaexitialis)是一种产于中国南方的剧毒蘑菇 .本研究首次用组织分离的方法获得了该菌的菌种。来自子实体上不同部位的组织都能用于分离 ,但用幼子实体上的菌褶分离效果最佳。试用六种培养基培养其菌丝体 ,其中以改良PDM为最佳。最佳培养温度为 2 6℃ ,最适pH值为 5 0 ,在 1 0 0 0mL的培养基中添加 2 0g葡萄糖对菌丝生长最为有利。菌丝产量在培养到70d时达到最大
【英文摘要】 Amanita exitialis Z.L.Yang & T.H.Li is a strongly poisonous mushroom occurring in south China,Its Pure culture was obtained for the first time via tissue isolation.tissues from different parts of the fruibody were available for isolation,but the gill of young fruitbody was the best.Six media were used to culture the mycelia.Among them,the improved PDM was the best.The optimal temperature for the mycelium growth was 26℃,and the optimal pH is about 5.0. The optimal consistency of glucose was 20g in 1 000 mL PDM.The output of the mycelia approached the highest after cultured for 70 days.
【中文关键词】 致命鹅膏; 菌种分离; 人工培养; 菌丝体
【英文关键词】 Amanita exitialis; Isolation; Pure culture; Mycelium
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,2005年02期 【DOI】CNKI:SUN:ZSYJ.0.2005-02-002