罗信昌
华中农业大学!湖北武汉430070
【中文摘要】 按照植物病理学原则 ,蘑菇分离物应依据柯赫法则 (Koch’spostulate)回接。在一定条件下 ,人工诱发子实体得以鉴别。但是一些蘑菇种类 (如某些共生食用蘑菇 )难以人工诱发子实体 ,即使能形成子实体也费时费力。本文介绍DNA指纹鉴别技术 ,即运用RAPD -PCR技术制成分离物及对应子实体指纹图谱 ,分析它们间DNA同源性及遗传相似度以鉴别分离物真伪 ,探求快速 ;简便 ;有效的鉴别方法。
【英文摘要】 The isolate of mushrooms should be identified by back inoculation,artificial induce sporophore formation under adapted condition according to Koch's postulate. However,it is very difficult to form sporophore by artificial inducing in some species,i.g.ectomycorrhizal mushrooms.Even if sporophore formation,it would take a long time and lots of efforts.Isolate would be identified by application PAPD-PCR technique,to make DNA band,analysis DNA homogeneity,DNA similarity coefficient between isolate and sporophore,which is of speed,handy and availble for characterization isolate of mushroom.
【中文关键词】 蘑菇; 分离物; RAPD-PCR; DAN图谱
【英文关键词】 Mushrooms; Isolate; RAPD-PCR; DNA band
【基金】国家自然科学基金!39770 0 2 5 ;; 高等学校博士学科点专项基金资助项目部分内容
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,2001年03期 【DOI】CNKI:SUN:ZSYJ.0.2001-03-000