尚德静; 王关林; 方宏筠
辽宁师范大学食品生化研究所; 辽宁大连生物工程研究所!辽宁大连116029; 辽宁大连生物工程研究所!辽宁大连116029
【中文摘要】 本文探讨了灵芝 (Ganodermalucidum)菌丝原生质体的制备及再生的条件。实验结果表明 :采用MYG为灵芝菌丝培养基 ,2 %真菌溶壁酶 (Lywallzyme)作脱壁酶 ,0 6mol/L甘露醇作渗透压稳定剂 ;对培养 6d的灵芝菌丝在 30℃下进行酶解 ,酶解时间 4- 6h ,可产生约 3 0× 1 0 6个 /mL (0 1g菌丝 )原生质体 ,并在添加灵芝子实体浸出汁的双层培养基上实现了灵芝菌丝原生质体的再生。
【英文摘要】 The conditions of protoplast formation and regeneration from the mycelia of Ganoderma lucidum were studied.Experimental results indicated that 3 0×10 6/mL protoplast of the mycelia of Ganoderma lucidum was obtained under optimal conditions,which are described as follows:Using 0 6mol/L mannitol as osmotic stabilizer;2% Lywallzyme as the enzyme digesting the cell wall;Using 6 days old MYG liquid cultures of the mycelia Ganoderma lucidum;at 30℃,enzyme digesting 4～6 hours.When incubated on two layer plates of agar,the protoplasts regenerated and formed new mycelia of Ganoderma lucidum.
【中文关键词】 灵芝菌丝; 原生质体
【英文关键词】 Mycelia of Ganoderma lucidum; Protoplasts
【文献出处】 中国食用菌,EDIBLE FUNGI OF CHINA,编辑部邮箱,2000年02期 【DOI】CNKI:SUN:ZSYJ.0.2000-02-000