刘标; 曹幼琴; 付庭治
南京大学生物科学与技术系
【中文摘要】 新分离到一株能产生较高水平菊粉酶的采绒革盖菌（ＣｏｒｉｏｌｕｓｖｅｒｓｉｃｏｌｏｒＺＱＷ－９６）。通过对培养基组成及培养条件的优化研究发现，用菊芋粉抽提液代替菊粉，氮源为玉米浆，添加０．０４％的吐温—８０的发酵培养基，在ｐＨ５．８、２４℃、转速为１５０ｒ／ｍｉｎ，装液量为７５ｍｌ／５００ｍｌ三角瓶条件下发酵６天后，胞外菊粉酶活性达２．２４ｕ／ｍｌ，比原来提高了１．３倍。关于该菌产菊粉酶的研究，国内外均未见报道
【英文摘要】 Coriolus versicolor strain ZQW-96 with higher inulinase activity was isolated. After studying of the optimal medium composition and culture conditions, we found that the strain's extracellular inulinase activity reached to 2 24u/ml with the medium whose carbon source was the extract of the tuber powder of Helianthus tuberosus instead of inulin, and nitrogen source was corn steep liquor, adding 0 04% Tween-80, and with the culture conditions was pH5 8, 24℃ 150r/min shaking, and six days' fermentation. This enzyme activity was 2 3 times higher than the initial. The report about the strain producing inulinase has never been found.
【中文关键词】 采绒革盖菌; 菊粉酶; 菊芋粉抽提液
【英文关键词】 Coriolus versicolor ZQW-96; Inulinase; Extract of the tuber powder of Helianthus tuberosus
【文献出处】 中国食用菌,EDIBLE FUNGI OF CHINA,编辑部邮箱,1997年03期 【DOI】CNKI:SUN:ZSYJ.0.1997-03-018