程南升; 潘学仁
东北林业大学林学系
【中文摘要】 比较了美味侧耳不同菌体组织中５种酶的同工酶谱变化。酯酶（Ｅ５Ｔ）和苹果酸脱氢酶（ＭＤＨ）在子实体中的酶带数和活性均高于液培菌丝。过氧化物酶（ＰＯＤ）的情况则正好相反。乙醇脱氢酶（ＡＤＨ）和山梨醇脱氢酶（ＳＯＤＨ）的酶带只在子实体中发现。酶谱随子实体部位而变异的进度同酶种类有关，ＰＯＤ变化较大，ＥＳＴ和ＭＤＨ较不明显，ＡＤＨ和ＳＯＤＨ没有变化。本实验结果为今后以这５种酶为生化遗传标记研究美味侧耳提供了基础依据。
【英文摘要】 Variation of isozyme patterns for five enzymes in different tissues of edible fungus PIeurotus sapidus was investigated,More isozyme bands and higher activity for esterase(EST)and malate dehydrogenase(MDH)were found in fruit body than in liquid-cultured mycelia,and the situation for perozidase(POD),however,was just on the con-trary.Activity of alcohol dehydrogenase(ADH) and sorbitol dehydrogenase(SODH) wasdetected only frops fruit body.The tissue specificity of isozyme patterns in fruit bodyvaried alnong the enzymes,most notable for POD,less for EST and MDH,and none forADH and SODH. The present test provided basis for further studies on Pleurotus sapidusconcerning the usage of these enzymes as biochemical or genetical markers.
【中文关键词】 美味侧耳; 同工酶; 组织特异性
【英文关键词】 Pleurotus sapidus; Isozyme; Tissue specificity
【文献出处】 中国食用菌,EDIBLE FUNGI OF CHINA,编辑部邮箱,1995年06期 【DOI】CNKI:SUN:ZSYJ.0.1995-06-014