金建康; 姚萍; 宋金娣; 王伟明; 丁风珍
江苏省农科院蔬菜所; 江苏省农科院蔬菜所; 江苏省农科院蔬菜所; 江苏省农科院蔬菜所; 江苏省农科院蔬菜所 南京210014; 南京210014; 南京210014; 南京210014; 南京210014
【中文摘要】 双孢蘑菇的常规杂交育种由于次级同宗结合而变得困难。本文研究了蘑菇原生质体制备与再生的最佳条件。从异核菌丝出发,制备与再生原生质体。根据自然性状,推定同核化再生株,栽培验证同核株,并根据配对行为确证同核株并分离异核化杂交种。本文认为原生质体杂交系统比常规杂交育种优越。
【英文摘要】 The routine hybridization of Agaricus bisporus is difficult for its secondarily homo- thallism.The Optimum preparat on and regeneration of protoplasts of this fungus were studied.Protoplasts were prepared and then regenerated from heterocayotic mycelium, homocaaryodized regenerates were presumed based upon the colonies,and then verified by cultivation fructuation,the authentic homocaryons was confirmed based oa the behavier of pairings,nearly 30 hybrids was eventually obtained.It's suggested that the protoplasty hybridization system is superior to the routine one.
【中文关键词】 双孢蘑菇; 原生质体; 同核化; 杂交系统
【英文关键词】 Agaric’us bispocus; Protoplast; Homocaryodization; Hybrdization system
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,1993年03期 【DOI】CNKI:SUN:ZSYJ.0.1993-03-012