【作者】赵竞男 苏晓庆
【机构】Department of Biology, Guiyang Medical College
【摘要】Pythium guiyangense is a mosquito pathogen, and has been proved to be a promising agent for biological control of mosquitoes. In order to develop the strains adaptable to different ecological environment having stable virulence to mosquito larvae, and being able to prolong the shelf life, an effort was made on transforming the fungus by using homologous or heterologous virulence genes. In this paper, a genetic transformation experiment of P. guiyangense mediated by Agrobacterium tumefaciens is reported. As a result, an A. tumefaciens mediated genetic transformation system was established successfully.
【基金】the National Natural Science Foundation of China (No. 30760140)； Guizhou Foundation for Resolving Key Scientific and Technique Problems [Contract No. Qiankehe NY (2006)3040]； Guizhou Foundation for International Scientific Co-operation [Contract No. Qiankehewai (2005) 400118 ]；