【作者】王澄澈 梁枝荣
【机构】河南省洛阳农业高等专科学校；中国科学院北京农村经济技术开发部；
【摘要】The protoplasts of auxotrophic mutant monokaryon of Pleurotus. sajor-caju was used as recipient parenL the protoplasts of wild dikareon of Lentinus edodes were heat-inactivated and used as nuclear donor parent. The fusants of two parents showed some their individual characteristics and physiological difference between themselfs. Thrugh protoplast re-isolation and reversion of one randomly selective fusant, two parents had been recovered and the donor parent achieved some new physiological characteristics such as faster mycelial growth and earier fruiting.This experiment result showed that the idea and the method of polarized protoplast fusion used in this study may be used as routine procedure for strain improvement in adible fungi.
【关键词】凤尾菇; 香菇; 原生质体; 非对称融合;