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DNA Fingerprinting of Ninety Wild Strains of Agaricus from China

2010-05-17 11:241770

CHEN Meiyuan1; LIAO Jianhua1; WANG Bo2; LI Hongrong1; LU Zhenghui1; GUO Zhongjie1; CAI Danfeng1; WANG Zesheng1 1Fujian Mushroom R&D Station; Edible Fungi Institute of Fujian Academy of Agricultural Sciences; Fuzhou; Fujian 350014; China; 2Soil and Fertilizer Institute of Sichuan Academy of AgriculturalSciences; Chengdu; Sichuan 610000; China

【中文摘要】 Ninety wild strains of Agaricus collected in China,including 44 strains preliminarily identified as Agaricus bisporus by isozyme electrophoresis,were studied using SRAP and ISSR techniques.Eighteen specific SRAP bands and 12 specific ISSR bands were scored,and a cluster dendrogram was constructed.The 90 wild strains of Agaricus were separated into two major groups,the first consisting of wild strains of A.bisporus and the second consisting of other Agaricus strains.Forty-one wild strains of A.bi...更多sporus collected in Sichuan and Tibet formed four groups according to the geographical location of the collection area.White A.bisporus strains from Xinjiang and Tibet(Xinjiang Wild,AgX04 and AgX042)exhibited unique band patterns resulting in lower similarity coefficient values(10%~13%)when compared with brown or light-brown A.bisporus strains.  还原

【英文摘要】 Ninety wild strains of Agaricus collected in China,including 44 strains preliminarily identified as Agaricus bisporus by isozyme electrophoresis,were studied using SRAP and ISSR techniques.Eighteen specific SRAP bands and 12 specific ISSR bands were scored,and a cluster dendrogram was constructed.The 90 wild strains of Agaricus were separated into two major groups,the first consisting of wild strains of A.bisporus and the second consisting of other Agaricus strains.Forty-one wild strains of A.bisporus collected in Sichuan and Tibet formed four groups according to the geographical location of the collection area.White A.bisporus strains from Xinjiang and Tibet(Xinjiang Wild,AgX04 and AgX042)exhibited unique band patterns resulting in lower similarity coefficient values(10%~13%)when compared with brown or light-brown A.bisporus strains.

【英文关键词】 Wild strains of Agaricus from China; SRAP; ISSR; cluster analysis
【基金】Supported by National Fund of Natural Sciences(No.30671456,30771503);; Key Science & Technology Programsof Fujian Province(No.2006S1001);; Fujian Fund of Natural Sciences(No.B0620004);; Key S&T InnovationItems of Fujian Academy of Agricultural Sciences(2007)

【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2009年01期 【DOI】CNKI:SUN:SYJB.0.2009-01-004

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