谢占玲; 何智媛; 唐龙清; 王学鲁; 朱海梅; 杨家华; 洒威
甘肃农业大学动物医学院; 青海大学生物科学系
【中文摘要】 以菌丝体生物量和胞外多糖量为指标,分别采用不同碳源和氮源对5株羊肚菌[Morchella angusticeps、M.conica、Morchella sp.(50647)、Morchella sp.(50648)和M.esculenta]进行液体发酵,筛选出这5株羊肚菌的最佳碳源、氮源以及最适合液体发酵的菌株。实验结果表明:5株羊肚菌最佳碳源均为可溶性淀粉,最佳氮源分别为:硫酸铵、蛋白胨、酵母膏、硝酸钠、硝酸钠。最适合液体发酵的菌株为Morchella angusticeps,其生长到第7天时菌丝体生物量最高,可达5.76g/L;第12天左右时胞外多糖产量最高,可达2.17g/L。
【英文摘要】 The effects of different carbon and nitrogen sources on mycelial biomass and extracellular polysaccharide production by five strains of Morchella grown in submerged culture are described.Soluble starch was the preferred carbon source for all five strains,whereas the preferred nitrogen sources for M.angusticeps,M.conica,Morchella sp.(strain 50647),Morchella sp.(strain 50648),and M.esculenta were ammonium sulfate,peptone,yeast extract,sodium nitrate and sodium nitrate,respectively.When all five strains were grown with their preferred carbon and nitrogen sources,highest mycelial biomass(5.76 g/L after 7 d)and extracellular polysaccharide(2.17 g/L after 12 d)yields were produced by M.angusticeps.
【中文关键词】 羊肚菌; 碳源; 氮源; 菌丝体生物量; 胞外多糖
【英文关键词】 Morchella spp; submerged culture; carbon source; nitrogen source; mycelial biomass; extracellular polysaccharide
【基金】青海省社会发展及基础研究资助项目(编号:Z-610)的部分研究内容
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2009年01期 【DOI】CNKI:SUN:SYJB.0.2009-01-010