冯翠萍; 程红艳; 唐兵; 张杰; 常明昌
山西农业大学食品科学与工程学院; 山西农业大学资源环境学院
【中文摘要】 采用超临界CO_2萃取法提取灵芝孢子油,通过正交试验确定最佳萃取条件是:萃取压力、温度、时间分别为25 MPa、45℃、60 min,CO_2流量25 L/h,在此条件下出油率可达3.96%。灵芝孢子油具有清除NaNO_2和DPPH(1,1-Diphenyl-2-picrylhydrazyl)自由基的作用,并且其清除作用随孢子油用量的增加而增加。
【英文摘要】 Orthogonal testing was used to optimize the conditions for extracting oil from Ganoderma lucidum spores using supercritical CO_2 fluid technology.Oil yields (3.96%) were highest under the following conditions:25 Mpa pressure,45℃extraction temperature,60 min extraction time and a CO2 flux of 25 L/h. Extracted spore oil was active in scavenging NaNO2 and DPPH (1,1-Diphenyl-2-picrylhydrazyl):NaNO_2 scavenging activity increased from 23.5% to 70.1% when the concentration of spore oil was increased from 1% to 5%,and DPPH scavenging activity increased from 20.2% to 40.5% when concentration of spore oil was increased from 1.25% to 10%.
【中文关键词】 超临界CO_2; 孢子油; 清除NaNO_2; DPPH自由基
【英文关键词】 supercritical CO_2; spore oil; NaNO_2 scavenging; DPPH radical
【基金】山西省科技攻关项目“珍稀菇类茶树菇、姬松茸以及灵芝的开发研究”(编号：021025)的部分研究内容
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2008年04期 【DOI】CNKI:SUN:SYJB.0.2008-04-018