忻雅; 阮松林; 王世恒; 马华升; 王伟科; 王淑珍
杭州市农业科学研究院
【中文摘要】 本研究利用RAPD标记和EST-SSR标记对10个不同来源的秀珍菇菌株进行聚类分析。200条RAPD引物中127条有扩增产物。引物可用率为63.5%。从NCBI数据库中下载秀珍菇及相近侧耳属食用菌EST序列2167条,聚类比对后得到全长为713.541 kb的非冗余EST 1442条,搜寻后得到的29个EST-SSR全部设计引物,其中26对引物(89.7%)显示多态性。根据17条RAPD核心引物和10对EST-SSR核心引物的扩增结果,对10个秀珍菇菌株进行了RAPD标记、EST-SSR标记及二者相结合的聚类分析。3种分析结果相近,且与菌丝、子实体生长特性分析结果相统一—10个供试菌株区分为5组:1～4号菌株为一组,6号和7号菌株为一组,8号和9号菌株为一组,5号和10号菌株各自为一组。
【英文摘要】 Amplification products were obtained from ten strains of Pleurotus geesteranus using 127 (63.5%) of 200 RAPD primers tested.A total of 2167 Expressed Sequence Tags (ESTs) from P.geesteranus and related Pleurotus species were downloaded from the NCBI database and,after clustering,1442 non- redundant ESTs with a total length of approximately 713,541 bp were obtained.Twenty-nine Expressed Sequence Tag- Single Sequence Repeats (EST-SSRs) were selected from these non-redundant ESTs and used to design EST-SSR primers.All 29 primer pairs were used for PCR amplification of genomic DNA extracted from the mycelia of ten P.geesteranus strains,and amplification products generated using 26 primer pairs (89.7%) exhibited polymorphisms.A dendrogram based on UPGMA (unweighted pair group method with arithmetic average) cluster analysis using combined data obtained using 17 RAPD core primers and 10 EST- SSR core primer pairs revealed that the 10 strains were clustered into five groups.
【中文关键词】 秀珍菇; RAPD标记; EST-SSR标记; 聚类分析
【英文关键词】 Pleurotus geesteranus; RAPD marker; EST-SSR marker; cluster analysis
【基金】杭州市珍稀食用菌科技创新服务平台(编号：20052212Y10)的部分研究内容
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2008年04期 【DOI】CNKI:SUN:SYJB.0.2008-04-008