肖建辉; 刘金伟; 刘祖林; 方宁; 肖瑜; 祁莹; 万卫红; 陈代雄; 梁宗琦
遵义医学院附属医院贵州省细胞工程实验室; 遵义医学院附属医院贵州省细胞工程实验室; 遵义医学院附属医院贵州省细胞工程实验室; 遵义医学院附属医院贵州省细胞工程实验室; 遵义医学院附属医院贵州省细胞工程实验室; 遵义医学院附属医院贵州省细胞工程实验室; 遵义医学院附属医院贵州省细胞工程实验室; 遵义医学院附属医院贵州省细胞工程实验室; 贵州大学真菌资源研究室 贵州遵义563003 ; 贵州遵义563003 ; 贵州遵义563003 ; 贵州遵义563003 ; 贵州遵义563003 ; 贵州遵义563003 ; 贵州遵义563003 ; 贵州遵义563003 ; 贵州贵阳550025
【中文摘要】 通过摇瓶液体培养实验,确定江西虫草培养基为蔗糖2%,甘油1%,黄豆浸液0.5%,酵母膏0.5%,KH2PO40.1%,MgSO40.05%,蒸馏水1000mL,初始pH6～7。装液量200～250mL 500mL,加入10粒分散球,按培养液量的5%～7.5%接种6d左右菌龄的菌种,于26～28℃、旋转式摇床150～180r min培养8d,最大菌丝体生物量可达16.6g L。
【英文摘要】 The culture conditions of Cordyceps jiangxiensis in shake flask culture were investigated. And the experiment results showed that the optimal compositions of submergod culture medium were as follows: sucrose 2%,glycerol 1%,soybean extract 0.5%,yeast extract 0.5%,KH_2PO_4 0.1%,MgSO_4 0.05%,distilled water 1000mL,initial pH 6～7. And the maximum mycelial biomass was as high as 16.6g/L when the medium capacity was 200mL～250mL in 500mL flask, 10 glass beads were used as dispersant, the seed of 6d age was inoculated at a rate of 5%～7.5%(V/V) and the flask culture experiment was carried out at 6～28℃ in a shake incubator with a rotation rate of 150r/min～180r/min for 8 days.
【中文关键词】 江西虫草; 菌丝体; 液体培养; 生物量
【英文关键词】 Cordyceps jiangxiensis; Mycelium; Submerged culture; Biomass
【基金】贵州省细胞工程重点实验室开放基金资助项目(No.02-003)
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2004年01期 【DOI】CNKI:SUN:SYJB.0.2004-01-006