詹才新; 凌霞芬; 杨家林
上海市农业科学院食用菌研究所农业部食用菌遗传育种重点开放实验室; 上海市农业科学院食用菌研究所; 鄂州市卫生专科学校 上海201106 ; 上海201106 ; 鄂州436000
【中文摘要】 以传统的形态 ,生理生化分析和最新的DCS -PDMA性亲和性测定方法为基础 ,结合群体分离分析和RAPD技术对来源于同一双孢蘑菇异核体菌株的 12个不育同核原生质体个体进行分析 ,筛选与性亲和性相关的分子标记。研究结果表明 ,供试的 12个不育同核原生质体个体被分成两大类性亲和性类型 ,其中一类 (A+ )包括不育同核原生质体个体B、C、D、E、F、G、H、I、J、L ,另一类 (A-)则仅仅包括不育同核原生质体个体K和M ,同时筛选到一个与性亲和性相关的分子标记OPA16 150 0 。从而为间接地利用双孢蘑菇本身特有的交配型作标记来指导杂交育种工作和进一步将其性亲和性基因定位分离克隆奠定了坚实的基础。
【英文摘要】 sterile homokaryotic protoplast isolates from the same heterokaryotic strain of Agaricus bisporus were analyzed in this paper. A molecular marker OPA16 1500 related to sexual compatibility was found using bulk segregation analysis and RAPD technology combined with the newest DCS-PDMA method and the traditional analysis of the morphological, physiological and biochemical characteristics of Agaricus bisporus. The results indicated that 12 sterile homokarytic protoplast isolates tested could be divided into two groups:group A + included B, C, D, E, F, G, H, I, J, L; group A - was made of K and M. Therefore, a solid foundation was established for Agaricus bisporus breeding through indirectly using its mating-type as marker for further location, isolation and cloning of its sexual compatibility gene.
【中文关键词】 双孢蘑菇; DCS-PDMA方法; 群体分离分析; 性亲和性相关分子标记; 杂交育种
【英文关键词】 Agaricus bisporus; DCS PDMA method; Bulk segregation analysis; Molecular marker of sexual compatibility; Cross breeding
【基金】国家自然科学基金项目 (95 - 0 1- 0 4 )
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2002年04期 【DOI】CNKI:SUN:SYJB.0.2002-04-000