胡国元; 朱兰宝; 周玉麟
湖北民族学院应用微生物研究所; 华中农业大学应用真菌研究所; 华中农业大学应用真菌研究所 恩施445000 ; 武汉430070 ; 武汉430070
【中文摘要】 采用垂直平板聚丙烯酰胺凝胶电泳,研究不同生长发育期、不同组织对金针菇[Fla?mmulina velutipes(Fr.)Kar.]黄、白品系酯酶同工酶电泳表型的影响,筛选出不受生长发育期及常规培养条件影响的酯酶标记区带.标记区带分基本带和识别带.酯酶同工酶标记区带电泳表型显示出多态型.利用酯酶同工酶标记区带分析黄、白金针菇酶谱差异,显示出黄、白金针菇品系有共同的遗传基础.
【英文摘要】 Orthogonal slab polyacrylamide gel electrophoresis (OSPAGE) was used to study the effect of different development stages and different tissues on the esterase isozyme electrophoresis phenotype of F. velutipes yellow and white strains. And marker bands of esterase isozyme which were not effected by different development stages and routine culture conditions were screened out. Esterase isozyme marker bands could be divided into primary esterase isozyme marker band which could be obtained from all tested strains, and distinguishing esterase isozyme marker bands which could only be found in some strains. It was proved that OSPAGE phenotype of esterase isozyme marker bands was polymorphic, and that using esterase isozyme marker bands to analyse the zymogram differentiation of F. velutipes yellow and white strains, the common genetic base was found to exist in the tested yellow and white strains.
【中文关键词】 金针菇; 黄色品系; 白色品系; 酯酶; 同工酶标记
【英文关键词】 Flammulina velutipes; Yellow strain; White strain; Esterase; Isozyme marker
【基金】农业部“八五”重点课题资助.
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,1997年04期 【DOI】CNKI:SUN:SYJB.0.1997-04-010