林树钱; 沈国华; 程立佳; 游兰英
福建省三明市真菌研究所; 福建省三明市真菌研究所; 福建省三明市真菌研究所; 福建省三明市真菌研究所 三明365000 福建省医学科学研究所 ; 三明365000 漳州市科委 ; 三明365000 ; 三明365000 厦门市医药研究所
【中文摘要】 本文研究了层卧孔菌[Fomitiporia punctata(Fr.)Murr.]的菌种分离、培养基配方.探索了培养条件,并投入中试规模的批量生产.层卧孔菌最适培养基为麦麸-蔗糖-酵母粉.中试生产温度控制在28±2℃,最适pH为5.5～6.0,最佳接种量为15%～20%.通气量为1:0.5(v/■v/min).培养80h左右.即可得到层卧孔菌菌丝体培养物.
【英文摘要】 Fomitiporia punctata (Fr. ) Murr. is one of the medicinal fungi used to treat coronary heart disease in Fujian Province. F. punctata strain was isolated, its medium composition and cultivation conditions in submerged culture were studied in order to produce the mycelia at pilot-production-scale. It was demonstrated that the optimum temperature, pH, inoculation rate and medium content per bottle were 28 + 2C, 5.56.0, 15%20% and 1 : 0.5 (v/v/min) resp. in pilot production, and that mycelium culture could be got after 80h cultivation.
【中文关键词】 层卧孔菌; 深层培养; 菌丝体培养物
【英文关键词】 Fomitiporia punctata (Fr. ) Murr; Submerged culture; Mycelium culture
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,1997年04期 【DOI】CNKI:SUN:SYJB.0.1997-04-008