路等学; 李成一; 段晓红; 王秉峰; 路晓玲; 李玉珍
甘肃省科学院生物研究所!兰州730000; 甘肃省科学院生物研究所!兰州730000; 甘肃省科学院生物研究所!兰州730000; 甘肃省科学院生物研究所!兰州730000; 甘肃省科学院生物研究所!兰州730000; 甘肃省科学院生物研究所!兰州730000
【中文摘要】 本研究以11个菌株为材料,对原生质体无性再生株的单、双核比例、产菇优势及出菇性状进行了研究。结果表明:（1）侧耳、香菇无性再生株以“锁状联合”的有无可区分为无锁状联合的单核株和有锁状联合的双核株,但其比例因菌株不同而迥异;（2）同一菌株的单、双核株的菌丝生长速度、出菇期及菇产量等性状有较大差异,双核株多优于单核株;但原生质体双核株与其亲株双核株相比,优劣因菌株而异;（3）无性单核株是进行细胞融合或常规杂交育种的理想材料,而原生质体双核株可用于选出优质高产菌株。
【英文摘要】 This study dealt with the ratio between the uninuclear and binuclear strains regenerated from the protoplasts of 11 strains of edible fungi, the yield superiority and their fruiting characters. The results showed that the regenerated strains from the protoplasts of the strains of Pleurotus and Lentinula edodes could be distinguished into the uninuclear strains without "clamp connection" and the binuclear strains with "clamp connection", but the ratio between the above strains was different, that, generated from the same parent strain, the uninuclear strain was markedly different from the binuclear strain in the growth rate of mycelia, fruiting period and yield etc, the binuclear strain was mostly superior to the uninuclear strain, but whether the binuclear strain was superior to the parental binuclear strain depended on the parent strain, and that the asexual uninuclear strain was ideal material for practicing cell fusion or routine breeding, but the protoplasmatic binuclear strain cou...更多ld be used for breeding high-quality and high-yield strains. 还原
【中文关键词】 侧耳; 香菇; 原生质体; 无性再生株
【英文关键词】 Pleurotus; Lentinula edodes; Protoplast; Asexual regenerative strain
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,1997年03期 【DOI】CNKI:SUN:SYJB.0.1997-03-000