戚淑威; 袁理春; 赵琪; 徐中志
云南省农业科学院高山经济植物研究所; 云南省农业科学院高山经济植物研究所; 云南省农业科学院高山经济植物研究所; 云南省农业科学院高山经济植物研究所 云南丽江674100; 云南丽江674100; 云南丽江674100; 云南丽江674100
【中文摘要】 研究了不同的配方培养基、pH值、温度对野生猪苓菌丝生长的影响。研究结果表明,25℃培养2d后,菌丝在黄豆饼粉培养基、玉米粉培养基、胡萝卜培养基以及苹果培养基上长满整个平皿,长势好,在其它培养基长势较差;菌丝在25℃、pH值为7.0的PDA培养基上生长较快,2d长满整个平皿,在pH值为5.0和6.0的培养基上生长慢,3d长满平皿,在pH值为8.0的培养基上生长很慢,10d长满平皿;温度为25℃时生长较快,2d长满平皿,15℃时3d长满平皿,5℃条件培养7d长满平皿。
【英文摘要】 Effect of different culture media,different pH and different temperature on the mycelium growing of wild Polyporus umbellatus was studied in this article.The results showed that the wild Polyporus umbellatus growimg covered the petri dishs at the culture medium which content bean flour,corn flour,carrot flour and apple flour respectively after 2 days incubation in the conditions of 25℃,the growth potential was very good,and the others were bad.The mycelium growing covered the petri dishs in the pH 7.0 PDA culture medium after 2 days incubation,however,in the pH5.0 and pH6.0 PDA culture media,its growing speed was slower than that in the pH7.0 PDA culture medium,and its growing speed was slowest in the pH8.0 PDA.It growing covered the petri dishs after 2 days incubation in the 25℃,in the 15℃,it needed 3 days to grow covered petri dishs,and needed 7 days to grow covered petri dishs in the 5℃.
【中文关键词】 猪苓; 组织培养; 培养基
【英文关键词】 Polyporus umbellatus; Tissue culture; Culture media
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,2007年05期 【DOI】CNKI:SUN:ZSYJ.0.2007-05-015