范雷法; 潘慧娟
浙江农科院园艺研究所; 浙江农科院园艺研究所 浙江杭州310021 ; 浙江杭州310021
【中文摘要】 本实验首先确定潮霉素浓度对香菇和平菇菌丝和原生质体再生的影响。其次进行了温度和时间对平菇原生质体灭活效果的研究。然后利用香菇和平菇再生原生质体对潮霉素抗潮霉素的差异,结合平菇原生质体灭活为融合子筛选依据,在融合剂诱导下获得4株融合子。
【英文摘要】 Hygromycin B resistance was studied on the mycelium and protoplast of Lentinula edodes and Pleurotus ostreatus.Test was done on inactivation of protoplast of P.ostreatus by temperature and duration.The protoplasts were prepared and fused with fusogen(PEG)after inactivation of protoplasts of Pleurotus ostreatus at 60℃ for 2h.Four fusants were obtained in the selection medium containning hygromycin B.
【中文关键词】 香菇; 平菇; 原生质体融合; 融合子; 潮霉素
【英文关键词】 Lentinula edodes; Pleurotus ostreatus; Protoplast fusion; Fusant; Hygromycin B
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,2005年05期 【DOI】CNKI:SUN:ZSYJ.0.2005-05-006