真菌完整染色体DNA的几种不同制备方法
Several Different Preparation Methods for Intact Chromosome DNA of Fungi
杨建明 冯爱萍 陈明杰 潘迎捷
摘 要：系统地介绍了目前用于真菌电泳核型分析的真菌完整染色体DNA的7种制备方法,并讨论了各种方法在真菌中应用的优缺点,为真菌电泳核型分析提供技术基础.
关键词：电泳核型; 完整染色体DNA; 原生质体法; 液氮研磨法; 直接包埋电泳法
分类号：Q949.32;Q343.2 文献标识码：A
文章编号：1005-9873(2003)02-0055-05
基金项目：本项目由上海市科学技术发展基金资助,项目编号(003912076)
作者简介：杨建明(1978-),男,2001年毕业于安徽技术师范学院生物系,学士学位,现为南京农业大学生命科学学院微生物学系在读硕士研究生.研究方向: 真菌遗传育种.陈明杰,本文通讯作者
作者单位：杨建明（南京农业大学生命科学学院,南京,210095）
冯爱萍（农业部食用菌遗传育种重点开放实验室;上海市农业遗传育种重点开放实验室;上海市农业科学院食用菌研究所,上海,201106）
陈明杰（农业部食用菌遗传育种重点开放实验室;上海市农业遗传育种重点开放实验室;上海市农业科学院食用菌研究所,上海,201106）
潘迎捷（农业部食用菌遗传育种重点开放实验室;上海市农业遗传育种重点开放实验室;上海市农业科学院食用菌研究所,上海,201106）
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