鹅膏菌属真菌RAPD分析及亲缘关系的研究
陈作红 张志光 张天晓
摘要：本文对采自湖南莽山的26种鹅膏菌属（Amanita）真菌进行了随机扩增多态性DNA （RAPD）分析，40个随机引物中筛选出扩增效果较好的6个引物，每个引物能产生1～10条DNA条带，获得的RAPD谱带清晰并呈现多态性OPG15、OPH04两个引物扩增的RAPD谱带能将26种鹅膏菌完全区分开来，通过6个引物的RAPD分析获得的平均相似性系数表明种与种之间的相关系数在20D60%之间，平均链锁聚类分析可将26种鹅膏菌分为二大类，且一些具菌环和苞状菌托的种类聚在一起，与形态分类基本相吻合。
关键词：鹅膏菌属， RAPD， 亲缘关系
中图分类号：Q939.5 文献标识码：A
文章编号：1007-3515（2000）01-0051-0055
STUDY ON RELATIONSHIP AMONG SPECIES OF
AMANITA USING RAPD
CHEN Zuo-Hong ZHANG Zhi-Guang ZHANG Tian-Xiao
(College of Life Science, Hunan Normal University, Changsha, 410081)
ABSTRACT: Random amplified polymorphic DNA (RAPD) was used in analysing the relationship among twenty-six speices of Amanita from Mangshan, Hunan. Of 40 primers, 6 ones generated reproducible RAPD profiles, and each one produced 1-10 bands. Two primers (OPG15 and OPH04) could differentiate 26 species of Amanita by its RAPD profiles. The similarity coefficients obtained from profiles generated by six primers among Amanita were about 20-60%. A dendrogram was constructed using average linkage clustering for clustering analysis. It indicated that there were two groups in 26 species and some species with ring and membranous volva clustered a group which coincided with morphological classification.
KEY WORDS: Amanita, RAPD, Relationship
国家科委重点课题资助项目93（7）-03
陈作红（湖南师范大学生命科学学院 长沙 410081）
张志光（湖南师范大学生命科学学院 长沙 410081）
张天晓（湖南师范大学生命科学学院 长沙 410081）
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