虫草多糖对日本沼虾免疫机能的影响
昌鸣先 陈孝煊 吴志新 胡先勤
摘 要：在饲料中添加1‰的虫草多糖，以口服形式对日本沼虾进行免疫。通过连续测定日本沼虾血淋巴吞噬活性，血清中抗菌活力、溶菌活力以及酚氧化酶活力，研究虫草多糖对日本沼虾免疫机能的作用。结果表明：除个别情况外，虫草组的吞噬百分比和吞噬指数、抗菌活力、溶菌活力以及酚氧化酶活力，均高于对照组。因此,投喂虫草多糖能够明显地增强日本沼虾的免疫防御能力。
关键词：免疫机能；虫草多糖；日本沼虾
分类号：S 963．73+6；S 945.4+9；S 942．5
The Effect of Cordyceps Polysaccharide on Macrobrachium nipponense Immune Function
Chang Mingxian（Huazhong Agricultural University, Wuhan 430070, China）
Chen Xiaoxuan（Huazhong Agricultural University, Wuhan 430070, China）
Wu Zhixin（Huazhong Agricultural University, Wuhan 430070, China）
Hu Xianqin（Huazhong Agricultural University, Wuhan 430070, China）
Abstract：Cordyceps polysaccharide, a product bought from med icine shops, with th e characteristics similar to those of β-glucan, was used as a food additive to test its immune effect. Macrobrachbium nipponense with a body we ight of 2.0～2.5 g were collected from the Dadongmen Aquaculture Market on Sept em ber 12, 2000，and nursed. Water temperature varied from 29℃ to 14℃ （n atural temperature），the water was aerated and changed once a day（1/2～2/3 volume of water was renewed each time）. The experiment of phenoloxidase （PO） test was designed as the same as those of bacteriolytic（Ul）and antibacterial （Ua）activity tests. Blood samples w ere c ollected from the haemocoel of 10～15 shrimps to measure PO, Ul, Ua activity on 0, 1, 4, 7, 14, 21 day. The phagocytosis was still measured after stopping the polysaccharide's feeding. The results showed that PO, Ua, Ul and phagocytosis of the test group are si gnif icantly higher than those of the controll group, indicating the effectiveness of cordceps polysaccharide on enhancing PO, Ua, Ul and phagocytosis of M. ni pponen se，leading to an increase in the immunnological activities. Further studies a re needed to identify the mechanism of cordceps polysaccharide in immune effect.
Keywords：immune function, Cordyceps polysaccharide, Macrob rachium nipponense
基金项目：武汉市重点科技项目
作者简介：昌鸣先，女，1972年生，华中农业大学水产学院硕士生．武汉 430070
作者单位：昌鸣先（华中农业大学水产学院，武汉 430070）
陈孝煊（华中农业大学水产学院，武汉 430070）
吴志新（华中农业大学水产学院，武汉 430070）
胡先勤（华中农业大学水产学院，武汉 430070）
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华中农业大学学报
JOURNAL OF HUAZHONG AGRICULTURAL UNIVERSITY
2001 Vol.20 No.3 P.275-278
