洛巴口蘑滤液中多糖蛋白复合物的抗肿瘤机制
ANTITUMOR MECHANISM OF POLYSACCHARIDE-PROTEIN COMPLEX FROM THE CULTURE FILTRATE OF TRICHOLOMA LOBAYENSE
刘方 黄容春 邢来君 赵力 张树庭
摘 要：从洛巴口蘑培养滤液中分离出一种具有抗肿瘤性的多糖蛋白复合物(PSPC).PSPC在细胞水平有能力恢复和增强带瘤鼠腹腔浸出细胞的吞噬功能和T细胞的促分裂活性.在分子水平,PSPC能恢复带瘤鼠脾细胞和腹腔浸出细胞内IFN-γ基因的表达水平.另外,PSPC能够下调带瘤鼠脾细胞内"抗细胞因子”TGF-β的表达水平.因此,PSPC的抗肿瘤机制可归咎于寄主介导的免疫调节作用.
关键词：多糖,蘑菇,细胞因子,免疫调节
分类号：Q939.93 文献标识码：A
文章编号：1007-3515-62000)03-0396-0400
作者单位：刘方（天津南开大学微生物系 天津 300071）
黄容春（香港中文大学生物系 香港）
邢来君（天津南开大学微生物系 天津 300071）
赵力（天津医院 天津 300211）
张树庭（香港中文大学生物系 香港）
参考文献：
［1］Abbas A K,Lichtman A H,Pober J S,1991.Cellular and Molecular Immunology.Philadelphia:W.B.Sannders
［2］Berg D J,Lynch R G,1991.Immune dysfunction in mice with plasmacytomas,I.Evidence that transforming growth factor-βcontributes to the altered expression of activation receptors on host B lymphocytes.The Journal of Immunology,146:2865～2872.
［3］Callard R E,1990.Cytokines and B Lymphocytes.London:Academic Press
［4］Chang R,1996.Functional properties of edible mushroom.Nutrition Reviews,54:91～93.
［5］Chang S T,Mao.X L,1995. Hong Kong Mushrooms.Hong Kong:The Chinese University of Hong Kong Press,79～80.
［6］Kehrl J H, Roberts A B,Wakefield L M,et al.,1986.Transforming growth factor beta is an important immunomodulatory protein for human B lymphocytes.The Journal of Immunology,137:3855～3860.
［7］Leung M Y,Fung P K,Choy Y M,1997.The isolation and characterization of an immunomodulatory and antitumor polysaccharide preparation from Flarmmulina velutipees.Immunopharmacology,35:255～263.
［8］Liu F,Ooi V E C,Chang S T,1995.Antitumor components of the culture filtrates from Tricholoma sp.World Journal of Microbiology and Biotechnology,11:486～490.
［9］Liu W K,Mak N K,Wong C C,1989.Immunomodulatory effect of methimazole on inbred mice.Immunobiology,18:23～32.
［10］iu W K,Ng T B,Sze S F,et al.,1993.Activation of peritoneal macrophages by polysaccharopeptide from the mushroom,Coriolus versicolor.Immunopharmacology,26:139～146.
［11］Mizuno T,Saito H,Nishitoba T,et al.,1995.Antitumor-active substances from mushrooms.Food Review International,11:23～61.
［12］Shibata Y,Tamura K,Ishida N,1983.In vivo analysis of the suppressive effects of immunosuppressive acidic protein,a type of alfa-1 acid glycoprotein,in connection with its high level in tumor-bearing mice.Cancer Research,43:2889～2896.
