阿魏蘑多糖理化性质及免疫活性研究
甘勇 吕作舟
摘 要：以阿魏蘑PleurotusferulaeLanzi子实体和菌丝体为试验材料，采用水浸法提取阿魏蘑多糖，分别得到子实体粗多糖A和菌丝体粗多糖B。将A经Sevag法去蛋白、透析、CTAB络合、乙醇沉淀、NaCl溶液溶解、透析，得到多糖A1。紫外光谱分析鉴定多糖A1为均一组分。苯酚-硫酸法测得多糖A1糖含量为82.9%。凝胶渗透色谱法测得多糖A1数均分子量Mn=141088，重均分子量Mw=142897。气相色谱分析多糖A1单糖组成及其摩尔比为Xyl∶Gla∶Glc=1∶1.102∶2.899。巨噬细胞吞噬作用试验、迟发型变态反应试验、白细胞介素-2（IL-2）的诱生与检测试验测得粗多糖A、粗多糖B具有免疫活性。
关键词：阿魏蘑，多糖，理化性质，免疫活性?オ?
分类号：Q939.96 文献标识码：A
文章编号：1007-3515（2001）02-0228-0232
STUDIES ON PHYSICAL AND CHEMICAL PROPERTIES AND IMMUNOLOGICAL ACTIVITIES OF POLYSACCHARIDES FROM PLEUROTUS FERULAE??
GAN Yong（The Laboratory of Applied Mycology, HuaZhong Agricultural University, Wuhan 430070）
LU Zuo-Zhou（The Laboratory of Applied Mycology, HuaZhong Agricultural University, Wuhan 430070）
Abstract：Crude polysaccharides A and B were extracted separately from the fruiting body and cultured mycelium of Pleurotus ferulae Lanzi with water-extracted method. For obtaining polysaccharide A1,protein was removed from crude polysaccharide A with Sevag method, dialysed with water, precipitated with CTAB and alcohol, dissolved with NaCl solution and then dialysed again . Identification of ultraviolet spectrum showed that the polysaccharide A1 was a homogeneous composition, and the purity of polysaccharide was 82.9% ,judging by the Phenol-H2SO4 method analysis. GPC (gel permeation chromatography)detection showed that the number average molecular weight of polysaccharide A1 was 141,088 and the weight average molecular weight of polysaccharide A1 was 142,897. GC (gas chromatography) analysis indicated the monosaccharides' composition and molar ratio of polysaccharide A1 were:Xyl∶Gla∶Glc=1∶1.102∶2.899.It was proved the crude polysaccharide A and B had immunological activities according to the experiments of macrophage phagocytosis, delayed type hypersensitivity and induction and detection of IL-2.
Keywords：Pleurotus ferulae, polysaccharide, physical and chemical property, immunological activity.
基金项目：湖北省重点科技计划（952P1007）资助项目
作者单位：甘勇（华中农业大学应用真菌研究室武汉 430070）
吕作舟（华中农业大学应用真菌研究室武汉 430070）
甘勇（山东鲁南制药集团，临浙 236000）
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