银耳的超声波介导转化
Transformation of Tremella fuciformis by Using Ultrasonic Mediation
谢宝贵 饶永斌 郑金贵
摘 要：以银耳(Tremellafuciformis)芽孢为实验材料,正交实验研究超声波处理时间、间歇时间及功率对转化率的影响.实验结果表明:超声波处理时间与间歇时间对质粒DNA和芽孢转化率都有显著影响;实验中质粒DNA转化率最高达到754.7个转化子/μg DNA,银耳芽孢转化率达到1.28‰;转化子的潮霉素抗性可达到200μg/mL,GUS检测阳性.
关键词：银耳;超声波介导转化;正交实验
基金项目：福建省科技项目(No.2001Z010)基金资助.
作者简介：谢宝贵:男,1962年生,博士,副教授.E-mail:＜xbg@pub2.fz.fj.cn＞.郑金贵:通讯作者.Author for correspondence.E-mail:＜jinggui@fjau.edu.cn＞.
作者单位：谢宝贵（福建农林大学生命科学学院,福州,350002;福建农林大学菌物研究中心,福州,350002）
饶永斌（福建农林大学生命科学学院,福州,350002;福建农林大学菌物研究中心,福州,350002）
郑金贵（福建农林大学农产品品质研究所,福州,350002）
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农业生物技术学报
JOURNAL OF AGRICULTURAL BIOTECHNOLOGY
2005 Vol.13 No.1 P.42-45
