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中国栽培白灵侧耳的RAPD和IGS分析

RAPD AND IGS ANALYSIS OF PLEUROTUS NEBRODENSIS CULTIVARS IN CHINA

张金霞　黄晨阳　张瑞颖　管桂萍　

摘　要：以RAPD和IGS为分子标记,研究了中国栽培白灵侧耳Pleurotus nebrodensis菌株的遗传多样性,RAPD结果表明,供试菌株间存在多态性;对供试菌株的IGS分析表明,白灵侧耳P.nebrodensis的IGSl区域较保守,菌株间没有多态性;IGS2区域进化较快,菌株间具丰富的多态性.IGS2-RFLP可应用于白灵侧耳的菌株鉴定鉴别,较RAPD更稳定,更具可操作性.

关键词：遗传多样性,菌株多态性,IGS2-RFLP

分类号：Q939.5 　文献标识码：A

文章编号：1672-6472(2004)04-0514-0519

基金项目：引进国际先进农业科学技术("948"计划)项目(2004-Z21)

作者单位：张金霞（中国农业科学院土壤肥料研究所,北京,100081）黄晨阳（中国农业科学院土壤肥料研究所,北京,100081）张瑞颖（中国农业科学院土壤肥料研究所,北京,100081）管桂萍（中国农业科学院土壤肥料研究所,北京,100081）　

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收稿日期：2004年5月24日

修稿日期：2004年7月1日

出版日期：2004年11月22日

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