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RNA复合物对松茸子实体的作用

2005-01-06 00:000

FFECT OF RNA RELATED COMPOUNDS ON FRUCTIFICATION OF TRICHOLOMA MATSUTAKE

Abstract:Ribonucleic acid and 5'-nucleotide were tested for fructification of Tricholoma matsutake in the field. Water solutions of RNAs were directly added to the mycelial colony of pine forest floor prior to fructification stage. Content of glucosamine, ergosterol and phospholipid of mycorrhizal fine roots were measured for estimation of mycelial status during growth and fructification stages. Phospholipid content was rapidly increased after RNAs addition. RNA solutions affected to make higher level of the mycelial components than control. As the final results, yield and number of T. matsutake fruit body were dramatically increased by the addition of RNAs during fructification period. This procedure was repeatedly done on the same plots three times in every fructification season, and resulted to all positive effect for RNAs supplement.

Keywords:mushroom, mycorrhiza, RNA stimulation additive

RNA复合物对松茸子实体的作用

大贺祥治  姚方杰  赵南爽  北本丰  李玉 

摘 要:施用核糖核酸和5'-核苷酸试验,其对生长于林间的松茸子实体的活化作用.在形成子实体之前将RNA关联物的水溶液直接洒入带有菌丝群落的松树林地表层.为了估计处于生长和形成子实体时期菌丝的状况对高菌根含量根中核苷、麦角固醇和磷脂的含量进行分析.与对照相比,RNA关联物的水溶液对提高核苷、麦角固醇和磷脂含量有作用,施用RNA关联物后9天磷脂含量迅速增加,RNA关联物有助于形成密集的菌丝群落.试验最后结果表明在形成子实体的两周时间内关联物使松茸子实体的产量和数量显著增加.

关键词:蘑菇,菌根,RNA刺激物

CLC Number:Q939.96  Document ID:A

Article ID:1672-6472(2004)04-0555-0562

Foundation Item:Grant-in-Aid for Scientific Research of Japan Society for the Promotion of Science (12660153).

Author Resume:姚方杰,Corresponding author: yaofj@yahoo.com.cn

Author Unit:大贺祥治(九州大学大学院农学研究院森林资源科学部门,福冈,日本,811-2415) 

     姚方杰(吉林农业大学园艺学院,长春,130118) 

     赵南爽(忠北大学林产工学科,清州,361-763,韩国) 

     北本丰(鸟取大学农学部生物资源科学,鸟取,680-0945,日本) 

     李玉(吉林农业大学菌物研究所,长春,130118,中国) 

References:

[1]Bligh E G, Dyer W J, 1959. A rapid method of total lipid extraction and purification. Can J Biochem Physiol, 37:911-917

[2]Braid G H, Line M A, 1981. A sensitive chitin assay for the estimation of fungal biomass in hard- woods. Holzforschung,35:10-15

[3]Dittmer J C, Wells M, 1969. Qualitative and quantitative analysis oflipids and lipid components. Methods Enzymol, 14:482-530

[4]Gerrits J P G, 1989. Supplementation of Agaricus compost with organic materials with special atten-tion to the uptake of minerals and amino acids. Mushroom Sci, 12:361-370

[5]Gold M H, Cheng T M, 1979. Conditions for fruit body formation in the white rot basidiomycetes Phanerochaete chrysosporium.Arch Microbiol, 121:37-41

[6]Hirato H, Kitamoto Y, 1995. Effect of physico-chemical characteristics of the culture substrate and nutritional conditions on mycelial growth in Tricholoma matsutake (in Japanese with English summary). Mushroom Sci Biotechnol, 2:17-22

[7]Inaba K, Ueda M, Takano Y, 1996. Screening of strains and media for artificial cultivation of Tricholoma matsutake (in Japanese). Abstracts of the 8th Annual Meeting of the Japanese Society of Mushroom Science and Biotechnology, 73

[8]Kawai M, Terada O,1976. Studies on the artificial reproduction of Tricholoma matsutake (S. Ito et Imai) Sing. Ⅱ Effects of vitamins, nucleic acid relating substances, phytohormones and metal ions in media on the vegetative growth of T. Matsutake (in Japanese with English summary). Trans Mycol Soc Japan, 17:168-174

[9]Ogawa M, 1983. How to Grow the Matsutake. Sobun, Tokyo. 1-232 (in Japanese)

[10]Ohga S, 1981. Biotechnological studies of edible mushroom cultivation Ⅲ. Promotion of growth of Lentinus edodes mycelium and antagonisms between Hypocrea sp. and Lentinus (in Japanese with English summary). Mokuzai Gakkaishi,27:136-140

[11]Ohga S, 1988. Biotechno1ogical studies of edible mushroom cultivation Ⅶ. Growth stimulating activity for Lentinus edodes by hot-water extracts of Allium fistulosum and nucleic acid-related compounds therein as the principle active components (in Japanese with English summary). Mokuzai Gakkaishi, 34:745-752

[12]Ohga S, Donoghue J D, 1998. Evaluation of methods for determining microbial activity and maturity of sawdust-based cultures of shiitake (Lentinula edodes). Mushroom Sci Biotechnol, 6:115-123

[13]Ohga S, Kudo M, 1992. Effect of various additives for the substrate maturity of Lentinula edodes. (in Japanese). Abstracts of the 36th Annual Meeting of the Mycological Society of Japan, 84

[14]Ohga S, Wood D A, 2000. Efficacy of ectomycorrhizal basidiomycetes on Japanese larch seedlings assessed by ergosterol assay.Mycologia, 92:394-398

[15]Ohta A, 1994. Production of fruit-bodies of a mycorrhizal fungus, Lyophyllum shimeji, in pure culture. Mycoscience, 35:147-151

[16]Ohta A, 1997. Ability of ectomycorrhizal fungi to utilize starch and related substrates. Mycoscience, 38:403-408

[17]Okamura T, Tani A, Sohgawa E, 1997. Effects of sake lees on the cultivation of the wild strain of Pleurotus ostreatus. Mushroom Sci Biotechnol, 5:29-32

[18]Oyama Y, Yoshida T, Taguchi H, 1974. The artificial cultivation of mycorrhiza-forming basidiomycetes. Mushroom Sci,9:719-731

[19]Seitz L M, Sauer D B, Burroughs R, 1979. Ergosterol as a measure of fungal growth. Phytopathology, 69:1202-1203

[20]Sekiya A, Magae Y, Uetake N, 1998. Utilization of erythritol in producing yeast for the cultivation of Pleurotus ostreatus (in Japanese with English summary). Mushroom Sci Biotechnol, 6:101-106

[21]Uno I, Ishikawa T, 1973. Metabolism of adenosine 3', 5'-cyclic monophosphate and induction of fruiting bodies in Coprinus macrorhizus. Bacteriol, 113:1249-1255

Manuscript Received:2004年5月31日

Manuscript Revised:2004年7月26日

Published:2004年11月22日

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